| Responsible Department | Department of Food Science | ||||||||||||||
| Course Dates | Week 25-26 | ||||||||||||||
| Course Abstract | Molecular biology techniques are increasingly used in Food Microbiology. These techniques allow for very specific species and strain identification, growth independent population determination and quantification, as well as quantification of gene expression. | ||||||||||||||
| Course Home Page | http://www.ifv.life.ku.dk/Aktiviteter/2012/molecul... | ||||||||||||||
| Course Registration | Filippa Bundgaard fbu@life.ku.dk | ||||||||||||||
| Deadline for Registration | 3rd of May 2013. Payment deadline 1 June 2013. | ||||||||||||||
| Credits | 6 (ECTS) | ||||||||||||||
| Level of Course | PhD course | ||||||||||||||
| Organisation of Teaching | Frederiksberg Campus. | ||||||||||||||
| Language of Instruction | English | ||||||||||||||
| Restrictions | Min 10, max. 16 participants. | ||||||||||||||
| Course Content | |||||||||||||||
| Statistical background for cluster analysis, and practical use of cluster analysis programme (Bionumerics). Introduction to public sequence databases and use of bioinformatics for design of PCR primers and probes. Species identification and strain typing using DNA finger-prints (16S rRNA gene sequencing, rep-PCR, PFGE). Culture independent detection and quantification of Food microorganisms (PCR and real-time PCR). Culture independent detection of diversity of microbial populations (DGGE/T-RFLP/sample tagged pyrosequencing). Expression of virulence genes under food-like conditions (Reverse-transcriptase real-time PCR). | |||||||||||||||
| Teaching and learning Methods | |||||||||||||||
| Lectures and practical exercises The course consists of a 10 workdays (8-17) theoretical and practical course and a theoretical project with relevance to the PhD project. Report for theoretical project must be delivered before 14/9-2013. | |||||||||||||||
| Learning Outcome | |||||||||||||||
| Molecular biology techniques are increasingly used in Food Microbiology. These techniques allow for very specific spe-cies and strain identification, growth independent popula-tion determination and quantification, as well as quantification of gene expression. The aim of the course is to give PhD students with limited molecular biology experience theoretical and practical knowledge in selected molecular biology techniques related to typing and identification, growth independent techniques and gene expression. | |||||||||||||||
| Course Literature | |||||||||||||||
| Curriculum will be uploaded app. 1 month before start of course. | |||||||||||||||
| Course Coordinator | |||||||||||||||
| Finn Kvist Vogensen, fkv@life.ku.dk, Department of Food Science/Food Microbiology, Phone: 353-33211 | |||||||||||||||
| Course Fee | |||||||||||||||
| PhD students: DKK 4,000 (you need proof from your supervisor of your PhD student status). Other participants: DKK 10,000. Fee covers lab costs, but does not include meals and accommodation. | |||||||||||||||
| Type of Evaluation | |||||||||||||||
| Participation in cource (80 % presence) and for PhD students preparation of thoretical report (2 week workload) (passed/failed). | |||||||||||||||
| Work Load | |||||||||||||||
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