LLEF10292 Dairy Microbiology

Details
Responsible DepartmentDepartment of Food Science

Earliest Possible YearBSc. 3 year to MSc. 2 year
DurationOne block
 
Credits7.5 (ECTS)
 
Level of CourseJoint BSc and MSc
 
ExaminationFinal Examination

oral examination


All aids allowed

Description of Examination: Description of Examination: Individual oral exam (20 min. incl. evaluation) general knowledge within starter and non-starter cultures and adventious microflora (including patogens and spoilage microorganisms) will be tested.

7-point scale, internal examiner
 
Requirement for Attending ExamPractical reports accepted
 
Organisation of TeachingLectures and practicals are throughout the available period. One excursion.
 
Block PlacementBlock 2
Week Structure: C
 
Language of InstructionEnglish
 
Optional Prerequisites270112 
Fermented Food and Beverages
 
Mandatory Prerequisites270017 
or 270112
 
RestrictionsNone
 
Course Content
The cource will contain lectures on:
Raw milk microflora.
Sweet dairy products microflora.
Pathogenic and spoilage microflora.
Primary (Lactic acid bacteria) and secondary (Yeasts, molds, propionibacterium, red smear microflora, LAB ripening cultures) starter cultures microflora.
Bacteriophage and bacteriophage resistance.
Role of starter culture physiology on growth and end products (aroma formation, proteolysis, glycolysis, amino acid catabolism)
Non-starter lactic acid bacteria, adjunct cultures and their role in cheese ripening.

Laboratory excersises will include:
Raw milk microflora
Charaterization of microflora in fermented dairy products using culture and culture independent techniques.
Detection and propagation of bacteriophages from dairy products.
Influence of starter culture propagation conditions on starter composition and activity.

An excursion to a starter company will be included.

 
Teaching and learning Methods
The course will have lectures and practical laboratory exercises throughout the 8 week period. Group report from laboratory exercise on microflora in fermented dairy products should be handed in and accepted. Practical laboratory exercises also complement previous knowledge obtained from 'Lactic Acid Bacteria Microbiology' or 'Fermented Foods and Beverages' An excursion to a starter company will be included.
 
Learning Outcome
The course is obligatory for the M.Sc. Dairy Science specialization. The aim of the course is to give the students a thorough knowledge of raw milk microflora, and microflora in sweet milk products as well as on microflora in starter cultures. Focus will be on primary and secondary starter cultures for dairy products, including the adventious microflora associated with cheese.

Through lectures and laboratory exercies the course will also give the students additional knowledge on microbiological analysis on organisms relevant for dairy products. Knowledge of the most important spoilage and patogens in dairy products has also been obtained.
After completing the course the student should be able to:
Knowledge:
Describe the composition of starter cultures and their use in dairy products. Describe the secondary, adjunct and adventious microflora found in cheese and their biochemical role in cheese ripening. Comprehend role of physiology of starter, secondary starter, adjunct cultures and non-starter bacteria in fermented dairy products. Comprehend principles for starter production. Display knowledge on culture dependent and culture independent techniqes for quantification of microorganisms from dairy products.
Skills:
Apply principles for species and strain identification of dairy organisms. Analyse dairy product for relevant microorganisms using classical and molecular biology based methods and evaluate the putative source of them. Compare literature information with own obtained data.
Competences:
Able to find, use and evaluate dairy microbiology literature in relation dairy fermentation processes.
Cooperate with fellow students about literature and practical laboratory work.
Communicate and evaluate own data in relation to literature in writing and orally.
 
Course Literature
S. Salminen, A. von Wright, & A. Ouwehand. Lactic Acid Bacteria, Microbiological and functional aspects. 3. ed. Marcel Dekker 2004 (selected chapters)

Reviews and Original litterature

Overhead and notes

Laboratory manuals
 
Course Coordinator
Finn Kvist Vogensen, fkv@life.ku.dk, Department of Food Science/Food Microbiology, Phone: 353-33211
 
Study Board
Study Committee LSN
 
Work Load
lectures28
preparation81
Excursions6
practicals75
examination1
supervision10
Colloquia5

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