| Ansvarligt institut | Institut for Jordbrug og Økologi
45 % Institut for Plantebiologi og Bioteknologi 45 % Institut for Basal Husdyr- og Veterinærvidenskab 10 % | ||||||||||||||
| English Title | Plant Genomics | ||||||||||||||
| Tidligst mulig placering | Bachelor 2. år til Kandidat 2. år | ||||||||||||||
| Varighed | En blok | ||||||||||||||
| Pointværdi | 7.5 (ECTS) | ||||||||||||||
| Kursustype | Fælleskursus | ||||||||||||||
| Eksamen | Sluteksamen skriftlig prøve Skriftlig auditorieeksamen Alle hjælpemidler tilladt Beskrivelse af eksamen: A four hours written examination Vægtning: 100 % 7-trinsskala, intern censur Eksamensdatoer: 22. juni 2012 | ||||||||||||||
| Forudsætninger for indstilling til eksamen | Accepted lab reports (75 %) and oral presentation of case | ||||||||||||||
| Rammer for Undervisning | Teaching includes a mix of lectures, laboratory and computer exercises complemented by cases for student groups. Laboratory work demonstrates selected key issues in plant genomics. Computer exercises demonstrate the potential of internet resourcesw | ||||||||||||||
| Blokplacering | Block 4 Ugestruktur: B | ||||||||||||||
| Undervisningssprog | Engelsk | ||||||||||||||
| Anbefalede forudsætninger | LBIF10184 Molekylær genetik | ||||||||||||||
| Begrænset deltagerantal | None | ||||||||||||||
| Kursusindhold | |||||||||||||||
| * Those contents marked with an asterisk will be offered as shared sessions between the Plant and Mammalian Genomics courses. The Big Picture: Introduction and Overview Crash course, Bioinformatics* and Crash course, Molecular genetics Genomes: Model plants versus crops Next-generation sequencing technologies* Gene expression Transcription factors, focus on MYB family From microarrays to next-generation sequencing (RNA-seq) Forward and reverse genetics Genetic markers and Quantitative Trait Loci Genotype - Phenotype; Map based cloning and QTL dissection Two very different plant genomics models: rice and moss Functional Genomics Metabolomics Biomarkers* and RNAi/siRNA* Student workshop on four functional genomics assignments Functional genomics debate on the findings of the case study In four practical exercises complementing the lectures, you will get familiar with the generation of haploid plant cell cultures, tracking of gene expression, both quantitatively as well as in plant tissue, analysis of quantitative trait loci, and genetic manipulation techniques. | |||||||||||||||
| Undervisningsform | |||||||||||||||
| 50 % for lectures and discussion classes. 50 % for practical exercises and supervised case studies. Students are expected to work in groups for their exercises and cases and written and oral presentations of their results are a requirement for admission to the final exam. | |||||||||||||||
| Målbeskrivelse | |||||||||||||||
| The course will provide basic understanding of the structure and evolution of plant genomes and central techniques used for studies of genomes and molecular breeding through a combination of lectures, cases, wet-lab exercises and computer exercises. Focus will be on the relationship between phenotypic traits and genotypes using the expanding information and resources on plant genomes and RNA/DNA/protein sequences. The course begins with the genomics and central techniques and databases developed for the two main plant model species, rice and Arabidopsis, and translates the principles to cultivated crops to understand the potential and constraints of applying genomic technology for plant breeding. After completing the course the students should be able to: Knowledge - Describe basic principles for the study of major model plants and general plant evolution. - Explain genetic markers and their use for qualitative and quantitative traits - Compare basic central experimental techniques used in plant genomics and molecular breeding and propose their application for novel challenges Skills - Complete with confidence an assement of the techniques used to study complex biological processes in plant model systems. - Practice molecular and genetic tools for plant improvement through molecular breeding of crops for food, fodder and production of high value crops for e.g. biomedicine, biofuel, green factories. - Manipulate cell cultures and conduct a simp0le transformation procedure. Competences - Evaluate various forward and reverse genomics approaches for gene isolation and functional studies. - Relate gene differences with phenotype by means of genomics - communicate effectively with other students in a debate to justify conclusions on a given problem. | |||||||||||||||
| Litteraturhenvisninger | |||||||||||||||
| Laboratory manuals and a dedicated compendium of selected scientific publications. Next to the compendium, no specific textbook will be required for this course. However, general botany and genetics textbooks may be consulted for the most basic concepts not covered within this course. Plant Genomics is an upper division course; the student should on his or her own initiative located additional reading material that matches the lecture material. | |||||||||||||||
| Kursusansvarlig | |||||||||||||||
| Søren K. Rasmussen, skr@life.ku.dk, Institut for Jordbrug og Økologi/Plante- og Jordvidenskab, Tlf: 353-33436 Björn Robert Hamberger, bjoernh@life.ku.dk, Institut for Plantebiologi og Bioteknologi/Plantebiokemi, Tlf: 353-33328 | |||||||||||||||
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