| Department of Disease Biology | |||||||||||||||
| Earliest Possible Year | MSc. 1 year | ||||||||||||||
| Duration | One block | ||||||||||||||
| Credits | 15 (ECTS) | ||||||||||||||
| Course Level | MSc Kurset er fælleskursus for studerende på fødevarevidenskab/Food Science and Technology | ||||||||||||||
| Examination | Final Examination written examination Written Exam in Lecturehall No aid allowed Description of Examination: 4 hours written exam. Weight: Written exam: 100% 7-point scale, internal examiner Dates of Exam: 30 October 2008 | ||||||||||||||
| Requirement For Attending Exam | Approved practical course and at least 75% attendance. Submission of report | ||||||||||||||
| Organisation of Teaching | lectures, problem discussions, practical laboratory course (amounts to nine full days) including report writing and problem solving | ||||||||||||||
| Block Placement | Block 1 Week Structure: A Block 1 Week Structure: B | ||||||||||||||
| Teaching Language | English | ||||||||||||||
| Optional Prerequisites | 230002 Biochemistry 1 240009 Cell Biology | ||||||||||||||
| Restrictions | min. 6 and max. 40 | ||||||||||||||
| Course Contents | |||||||||||||||
| The theoretical part of the course will be both basic immunological principles and also give an updated version of molecular immunology. Included will be enlightenment lectures from laboratories of Virology, Bacteriology and Parasitology (know your foe) - which will also provide information on possible graduate research projects. The practical course will be focused on three main themes: 1. Immunochemistry 2. Molecular Biology 3. Immunohistochemistry/-cytochemistry 1. Immunochemical methods for antibody purification and labelling. Purified protein will be analyzed by SDS-PAGE and used for Western blot. Also various quantitative and qualitative methods will be used to demonstrate the versatility of antibodies - all excercises provide a context with the theoretical part. 2. The students will also use molecular methods to clone a gene encoding a tissue antigen and will finally be able to obtain sequence and produce a basic identification and genomic mapping using www based programs. 3. Detection of viral infection in cell cultures/tissues using antibody reagents. (courtesy of lab for Virology) The practical course include the writing of a short report and answering questions stated in the manual. | |||||||||||||||
| Teaching And Learning Methods | |||||||||||||||
| Lectures, problem solving, practical course (nine day incl. report writing). | |||||||||||||||
| Learning Outcome | |||||||||||||||
| The objective is to provide an updated knowledge of basic cellular and molecular innate and adaptive immunology, as well as some clinical subjects. The practical course will apply a variety of immunological and DNA-techniques. After the course the student will be able to Knowledge: Describe pathogen recognition by pattern-recognition-receptors, creation of inflammation and TLR-receptor signalling as initiators of adaptive immunity. Describe the structure and function of B-cell and T-cell receptors at the cell and protein levels, as well as the creation of the receptor repertoire at the gene level. Describe the genetics and structure of MHC molecules and the main antigen processing pathways leading to T-cell activation and effector functions. Summarise the general principles of signal transduction. Describe the development of lymphocytes and tolerance induction in primary lymphoid organs. Define the main functional differences between the most important T-cell and B-cell subsets and creation of memory. Describe the main special features of mucosal immunity. Describe examples of pathogen evasion and subversion of immune defences and be able to describe mechanisms and causes of some immunodeficiency diseases, hypersensitivity, autoimmunity and main causes of problems in translantation immunology. Describe various strategies on how the immune system can be manipulated to avoid unwanted immune responses, fight tumours and main vaccination strategies. After the practical course the student will Have knowledge of and be able to use main analytical methods involving antibodies (use as detection reagent, structure elucidation, antigen quantification) as well as gene cloning and the use of www for bioinformatics to solve immunological questions. Be able to explain and describe main effector mechanism of immunity when an organism is challenged by various pathogens - from the onset and until clearance. Skills: Use immunologically founded reasoning on various scientific problems concerning health and related issues. Use various immunologically and molecular techniques to solve questions raised in immunology and related fields. Competences: Have acquired knowledge to discuss immunological literature, immunological problems, techniques and an updated knowledge of molecular immunology - both basic and more specialised areas. Have a technical foundation to suggest experimental analysis of some immunological questions. | |||||||||||||||
| Course Litterature | |||||||||||||||
| Janeway, Travers, Walport, Shlomchik: immunobiology. 2005. 6ed Garland. J. Salomonsen and B. Aasted: manual for practical course in Immunology. | |||||||||||||||
| Course Coordinator | |||||||||||||||
| Jan Salomonsen, jas@life.ku.dk, Department of Veterinary Pathobiology/Section for Virology, Immunology and Fish diseases, Phone: 35333128 | |||||||||||||||
| Study Board | |||||||||||||||
| Study Committee NSN | |||||||||||||||
| Course Scope | |||||||||||||||
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