| Inst. for Grundvidenskab og Miljø | |||||||||||||||
| English title | Enzymology and Experimental Biochemistry | ||||||||||||||
| Tidligst mulig placering | |||||||||||||||
| Varighed | En blok | ||||||||||||||
| Pointværdi | 7.5 (ECTS) | ||||||||||||||
| Kursustype | Kandidatkursus | ||||||||||||||
| Eksamen | Sluteksamen skriftlig og mundtlig eksamen Alle hjælpemidler tilladt Beskrivelse af eksamen: Evaluation of reports of performed experiments supplemented with individual oral presentation and examination Vægtning: Oral examination in project report and pensum is judged by 100 % 7-trinsskala, intern censur | ||||||||||||||
| Forudsætninger for indstilling til eksamen | Submission of at least 75 % of project reports | ||||||||||||||
| Rammer for undervisning | The course consist of a combined theoretical part (lectures and theoretical exercises; approx. 6 h per week) and practical laboratory work (approx. 9 h per week) with traditional and new advanced methods of analyses used in work with biomolecules. | ||||||||||||||
| Blokplacering | Block 2 Ugestruktur: A | ||||||||||||||
| Undervisningssprog | Engelsk | ||||||||||||||
| Anbefalede forudsætninger | 230002 Biokemi 1 Biochemistry 1 | ||||||||||||||
| Begrænset deltagerantal | The number of students per course is limited to 30 owing to limited space in the laboratories and the teaching in use of advanced instrumentation. | ||||||||||||||
| Kursusindhold | |||||||||||||||
| The course comprises the theoretical and experimental work associated with studies and uses of biomolecules as bioactive proteins, peptides, and enzymes occurring in biological matrices of plant, animal, and microbial origin. It is especially molecules of relevance for quality of feed, food, and non-food products,as well as those involved in metabolic regulations associated with health and diseases, and enzymes used in analyses and bioprocessing technologies. Focus is placed on understandings of the relations between structure and properties of the molecules, as well as the effects of ions, organic molecules and surfactants on the properties of water as solvent, extraction, and chromatographic systems. The combinations of such knowledge give the students basis for understanding and development of a relevant strategy of methods as used in analytical biochemistry. The students will in selected projects work with both traditional and new actual /advanced biochemical methods and techniques. This give possibilities for knowledge to and experiences in applied biochemical research, quality assurance, Good Laboratory Practice (GLP) etc as required in basic research laboratories, in biotech companies, industries, applied sector research institutes and control laboratories. The course gives knowledge to: · Quality assurance including GLP in enzymology and experimental biochemistry · The theoretical and experimental based knowledge to changes in water as solvent, extraction and chromatographic systems, as function of ions, organic modifiers and surfactant type and concentration · Thermodynamics and kinetics in enzymology and biochemical analyses · Binding between molecules in equilibrium reactions and thermodynamic stable complexes. · Native enzymes: holoenzymes, apoenzymes, co-factors, activation energy and kinetics · Receptor and effector sites, positive (activators) and negative effectors (inhibitors) and their effects on the active sites · Strategies to efficient isolation, purification and characterisation of biomolecules, bioactive proteins, peptides, and enzymes from complex biological systems · Determination of kinetic parameters, Vmax, KM and kcat, and interpretation of such data in relation to ideal catalytic effects · Knowledge to new and traditional biochemical methods and experimental experiences in use of such methods · Use of enzymes in analytical methods, in feed, food and bioprocess technologies, in xenobiotic metabolism, and diagnose of health and disease effects The individual projects for each course participant include use of original scientific literature, its interpretation, evaluation and discussion in relation to results obtained in the project. | |||||||||||||||
| Undervisningsform | |||||||||||||||
| Lectures and theoretical exercises: lectures will be used to present the theoretical background for the subjects considered with at least 2 hours per week and folowing the progress in the study program and experimental work. The lectures will be followed by theoretical exercises where the students in groups of three will work on answers of specific questions related to the theory and practical use of methods and technology. Laboratory work with knowledge to theory and practical use of the various instruments and biochemical methods are central parts of the course. The students will be in groups of three and spend about 9 hours per week with this part of the course. The last 2/3 of the course period will be devoted to a larger project focused on studies of a defined bioactive molecule. The project on such a defined molecule is chosen among a selection of high molecular weight biomolecules, and agreed on by the student and the teacher. The combined theoretical and practical laboratory experiments with focus on strategies of methods in analytical biochemistry and enzymology will train the students in calculations on the produced data, statistical analyses of obtained results, performance of good laboratory practise (GLP), quality assurance, and reporting with evaluation of own data in relation to literature data. | |||||||||||||||
| Målbeskrivelse | |||||||||||||||
| The course provides an advanced theoretical and practical basis for knowledge to and work with enzymes occurring in complex biological matrices as plant-, animal- and microbial based feed and food. Focus is placed on the chemical relations between structure and properties of biomolecules, theoretical and experimental strategies in use of actual new and traditional biochemical methods and understanding of the descriptions in original scientific papers. After completing the course the student should be able to: Knowledge: Reflect about physico-chemical-biochemical properties of biomolcules and especially enzymes. Describe strategies to efficient isolation and purification of enzymes from complex biological systems Describe analytical techniques used in biochemistry and for enzyme characterisation. Understand the theoretical basis for changes of water as solvent, extraction and chromatographic systems in work with molecules of different polarity. Display overview of changes in thermodynamic constants to actual or practical constants as function of compounds changing the properties of pure water and diluted solutions. Skills Evaluate the detection, quantification and linearity of experimental data in relation to published values (literature data). Design experimental stategies in analytical biochemistry and enzymology based on structure and properties of analytes in complex matrix systems. Apply theoretical principles of analytical biochemistry to carry out experimental isolation and purification of enzymes Evaluate the performance of applied techniques in an enzyme related project carried out in a group (preferably 2-3 persons). Competences Cooperate with a fellow student on laboratory reports and a project report Expand the knowledge to biochemistry by use of mathematics and statistical evaluations Utilise the obtained knowledge in more comprehensive projects within research, development or industry. | |||||||||||||||
| Litteraturhenvisninger | |||||||||||||||
| R.K. Scopes (1994): Protein purification. Principles and Practice. Springer-Verlag New York Berlin Heidelberg (ISBN 0-387-94072-3) B.W. Wenclawiak, M. Koch, W. Hadjicostas (Eds) (2004): Quality Assurance in Analytical Chemistry. Training and Teaching. Springer - Verlag, Berlin, Heidelberg. ISBN 3-540-40578-X. L Buckberry & P. Tessdale (2001): Essenstials of Biological Chemsitry (udvalgte afsnit). John Wiley & Sons Ltd. West Sussex, UK (ISBN 0-471-48906-9) H.Sørensen, S.Sørensen, C.Bjergegaard, S.Michaelsen (1999): Chromatography and Capillary Electrophoresis in Food Analysis. Royal Society of Chemistry, Cambridge, UK. (ISBN 0-85404-561-9)(udvalgte afsnit) Selected scientific literature relevant for projects | |||||||||||||||
| Kursusansvarlig | |||||||||||||||
| Jens Christian Sørensen, jchs@life.ku.dk, Institut for Grundvidenskab og Miljø/Biokemi og naturproduktkemi, Tlf: 35332435 | |||||||||||||||
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