| Department of Veterinary Pathobiology | |||||||||||||||
| Earliest Possible Year | MSc. 1 year | ||||||||||||||
| Duration | One block | ||||||||||||||
| Credits | 7.5 (ECTS) | ||||||||||||||
| Course Level | MSc | ||||||||||||||
| Examination | Final Examination written examination Written Exam in Lecturehall No aid allowed Description of Examination: 4 hours written exam. Weight: Written exam: 100% 13-point scale, internal examiner Dates of Exam: 28 January 2006 | ||||||||||||||
| Requirement For Attending Exam | Approved practical course and at least 75% attendanceel. Submission of report | ||||||||||||||
| Organisation of Teaching | lectures problem discussions practical laboratory course (amounts to six full days) including report writing and problem solving | ||||||||||||||
| Block Placement | Block 2 Week Structure: A | ||||||||||||||
| Teaching Language | English | ||||||||||||||
| Optional Prerequisites | 035218,057611 | ||||||||||||||
| Restrictions | 40 | ||||||||||||||
| Areas of Competence the Course Will Address | |||||||||||||||
| Competences obtained within Basic Science: Knowledge of how lymphocyte receptor diversity is created and antigen recognized. Understanding the regulation of immune responses in various compartment of the organism. Comprehend the interaction between innate and adaptive responses. Competences obtained within Applied Science: Knowledge of a variety of animal models, as well as cellular and molecular techniques used for understanding the immune system. Comprehend the resolution of assorted immunochemical techniques. Appreciate the versatility of antibody mediated techniques in molecular biology in general. Apply and understand technical principles in the practical course. Competences obtained within Ethics and Values: Is aware of beneficial and controversial aspects of stem cell research, transgenesis, xenotransplantation, etc. | |||||||||||||||
| Course Objectives | |||||||||||||||
| Students will learn basic immunology at the molecular level. The practical course introduces a variety of antibody mediated methods, as well as the use of DNA techniques for answering immunological questions The outcome should be an understanding of the components and the biology of the immune system, at the level of population, organism, cell, protein and gene. Students should also appreciate immunology as a discipline offering multiple tools for use in other areas of biology. | |||||||||||||||
| Course Contents | |||||||||||||||
| The theoretical part of the course will be both basic immunological principles and also give an updated version of molecular immunology. The practical part of the course will use immunochemical methods for antibody purification and labelling. Purified protein will be analyzed by SDS-PAGE and used for Western blot. Also various quantitative and qualitative methods will be used to demonstrate the versatility of antibodies - all excercises provide a context with the theoretical part. The students will also use molecular methods to clone a gene encoding a tissue antigen and will finally be able to obtain sequence and produce a basic identification using www based programs. The practical course include the writing of a short report and answering questions stated in the manual. | |||||||||||||||
| Teaching And Learning Methods | |||||||||||||||
| Lectures, problem solving, practical course (six day incl. report writing):purification of immunoglobulin. Quality and quantity determination.Use as reagent. Also use DNA methods for tissuetyping animal:from PCR to DNA sequence and database searches/mining | |||||||||||||||
| Course Litterature | |||||||||||||||
| Janeway, Travers, Walport, Shlomchik: immunobiology. 2005. 6ed Garland. J. Salomonsen and B. Aasted: manual for practical course in Immunology. | |||||||||||||||
| Course Coordinator | |||||||||||||||
| Jan Salomonsen, jas@life.ku.dk, Department of Veterinary Pathobiology/Section for Virology, Immunology and Fish diseases, Phone: 35333128 | |||||||||||||||
| Study Board | |||||||||||||||
| Study Committee NSN | |||||||||||||||
| Course Scope | |||||||||||||||
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