| Department of Agricultural Sciences | |||||||||||||||
| Earliest Possible Year | |||||||||||||||
| Duration | 1 semester | ||||||||||||||
| Credits | 9 (ECTS) | ||||||||||||||
| Course Level | Joint BSc and MSc Joint BSc and MSc level | ||||||||||||||
| Examination | oral examination Aid allowed Description of Examination: pass/fail, internal examiner 70% of mark based on project report Description of Examination: 30% of mark based on oral examination | ||||||||||||||
| Organisation of Teaching | Discussion classes, project work in groups | ||||||||||||||
| Block Placement | E SUP F SUP According to agreement | ||||||||||||||
| Teaching Language | English may be conducted in Danish | ||||||||||||||
| Optional Prerequisites | 055411 Plant Breeding 010611 Statistics, Basic Course Plante Breeding; Statistics, Basic Course | ||||||||||||||
| Course Objectives | |||||||||||||||
| The aim of the course is to provide a practical as well as a theoretical introduction to basic biotechnology for plant breeding. Based on acquired basic skills, students are introduced to further principles of planning, management, and report writing for biotech projects. | |||||||||||||||
| Course Contents | |||||||||||||||
| A general introduction to major techniques and principles of plant breeding biotechnology is given during the first semester, comprising: * Micropropagation. Germplasm establishment in vitro, multiplication techniques, in vitro storage, and re-establishment in soil. * Callus and cell suspensions. Cell culture establishment, maintenance and plant regeneration. * Haploid production. Anther/microspore culture, chromosome elimination, and ovule/ovary culture. * Protoplast technology. Protoplast isolation, culture, fusion, and regeneration of plants. * Genetic transformation. Agrobacterium mediated transformation, direct gene uptake including biolistic systems. * Molecular markers. RFLP, RAPDS, AFLPs, and microsatellites. Map construction, QTL-mapping, and positional cloning. | |||||||||||||||
| Teaching And Learning Methods | |||||||||||||||
| The course is comprised of three parts: A basic part, supervised exercises, and a project. During the basic part, which lasts one month, the students fundamental skills are established. This enables them to perform independent design and preparation of culture media, sterilisation and dissection of explants, as well as other laboratory operations, based on descriptions from the literature. During the two months of supervised exercises, students conduct experiments within the major topics: micropropagation, cell culture, haploids, protoplast and transformation, and molecular markers. Supervised exercises use plant material prepared in advance and prepared structural protocols to be completed by the students. Part of the first semester is spent planning the course project based on the students ideas and literature searches. The second semester is devoted to the experimental project with plant material and follows the plans prepared by the students. A final written report based on the course project is used for the final exam. | |||||||||||||||
| Course Litterature | |||||||||||||||
| Bajaj, Y.P.S. (1986-95): Biotechnology in agriculture and forestry. Vols. 1-33. Springer-Verlag, Berlin Heidelberg New York George, E.F. & P.D. Sherrington (1984): Plant propagation by tissue culture. 709 pp.; Jain, S.M., S.K. Sopory & R.E. Veilleux (1996-7): In vitro haploid production in higher plants. Vols. 1-5. Kluwer Academic Publishers. | |||||||||||||||
| Course Coordinator | |||||||||||||||
| Sven Bode Andersen, sba@life.ku.dk, Department of Agricultural Sciences/Plant and Soil Science, Phone: 35333444 | |||||||||||||||
| Study Board | |||||||||||||||
| Study Committee AHJ | |||||||||||||||
| Course Scope | |||||||||||||||
| |||||||||||||||